Characterization of the Catalytic Subunit of Factor XIII
نویسنده
چکیده
Plasma factor XIII is composed of two subunits, a and b, whereas platelet and other intracellular zymogens have only a-subunits. The catalytic subunit, a. is the same in all forms. In order to characterize the interactions of awith b-chains in the plasma zymogen and a-chains with other molecules and to correlate factor XIII activity with aprotein. a specific, sensitive radioimmunoassay was developed. With the polyclonal antisera used, the assay recognizes all molecular forms of a (zymogens, activation intermediates. enzyme) equally well. The assay can be used to determine a-chain concentration in plasma and serum and in purified test systems. Fibrinogen in high concentrations
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تاریخ انتشار 2005